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| Registros recuperados: 79 | |
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| Lallias, Delphine; Beaumont, A.r.; Haley, C.s.; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie. |
| This study presents the first genetic linkage map for the European flat oyster Ostrea edulis. AFLP and 20 microsatellite markers were genotyped in a three-generation pedigree comprised of 2 grand-parents, 2 parents and 92 progeny. Chi-square goodness of fit tests revealed high segregation distortion, which was significant for 32.8% of markers. Sixteen microsatellites and 235 AFLPs (170 AFLPs type 1:1 and 65 AFLPs type 3:1) were used to build sex-specific linkage maps using CriMap software. The first parental map (P1) consisted of 104 markers grouped in nine linkage groups, spanning 471.2 cM with an average spacing of 4.86 cM. The second parental map (P2) consisted of 117 markers grouped in ten linkage groups (which equals the haploid chromosome number),... |
| Tipo: Text |
Palavras-chave: Microsatellite; AFLP; Ostrea edulis; Flat oyster; Genetic linkage map. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2642.pdf |
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| Lallias, Delphine; Lapegue, Sylvie; Harley, Crhis; Boudry, Pierre; Heurtebise, Serge; Beaumont, Andy. |
| The flat oyster Ostrea edulis is the species endemic to European coasts, both Atlantic and Mediterranean. It has been exploited since Roman times, but its aquaculture production decreased from around 20000 tons in the 1950's to 1500 tons today because of two successive diseases due to the intracellular parasites Marteilia refringens and Bonamia ostreae. Since 1985, Ifremer has been undertaking a breeding program to produce families of oysters tolerant to Bonamia. In this context, a further step would be to identify QTLs for resistance to this parasite. Therefore, as a first step a genetic map is now being built. The establishment of a genetic linkage map will provide a foundation for the mapping of QTLs, with the ultimate objective to implement... |
| Tipo: Text |
Palavras-chave: QTLs; Ostrea edulis; Flat oyster; Genetic linkage map; Genetic. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3466.pdf |
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| Boudry, Pierre; Naciri, Yamama; Collet, Bertrand; Cornette, Florence; Heurtebise, Serge; Ledu, Christophe; Chollet, Bruno; Phelipot, Pascal; Gerard, Andre. |
| Les objectifs pour l'année 1996 étaient les suivants: Poursuivre les premières reproductions des espèces pour lesquelles nous disposons de géniteurs GO. L'objectif est toujours de limiter le stockage d'animaux importés en produisant une descendance simultanément à des témoins C. gigas qui serviront de comparaison. Tenter des hybridations inter-spécifiques des différents taxons présents au sein du conservatoire en fonction des connaissances disponibles dans la littérature sur ce sujet (voir pour revue Gaffney et Allen, 1993). • Importer de nouvelles souches ou espèces. Élargir la gamme des espèces présentes au sein du conservatoire de souches (les capacités d'accueil de la salle de quarantaine étant Limitée). |
| Tipo: Text |
Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea; Sélection génétique; Saccostrea commercialis. |
| Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00044/15516/12903.pdf |
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| Boudry, Pierre; Naciri, Yamama; Launey, Sophie; Ledu, Christophe; Phelipot, Pascal; Heurtebise, Serge; Gerard, Andre. |
| Le laboratoire IFREMER "Génétique Aquaculture et Pathologie" (GAP) (La Tremblade et Bouin) a entrepris des actions de recherche sur ces différents points. L'Unité de Recherche en Génétique, en collaboration avec tous Ies membres du Réseau Génétique Mollusques (REGEMO) a axé une partie de ses travaux sur l'acclimatation et l'hybridation de différentes espèces du genre Crassostrea. Ces recherches ont débuté avec le soutien du Conseil Général de Charente-Maritime depuis 1992 sur des essais d'acclimatation de C. virginica, l'huître creuse américaine. En prolongement de cette étude, l'URGE a proposé à la région Poitou Charentes un programme de recherche plus vaste sur l'ensemble des espèces d'huîtres creuses. Le contrat de plan Etat-Région a été signé en... |
| Tipo: Text |
Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea; Saccostrea; Génétique population. |
| Ano: 1994 |
URL: http://archimer.ifremer.fr/doc/00044/15517/12904.pdf |
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| Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre. |
| Dans le cadre du plan Etat-Région 1994-1998, un programme de conservatoire de souches a été initié au Laboratoire IFREMER de La Tremblade. Ce conservatoire a pour objectif d'acclimater différentes espèces ou souches d'huîtres creuses (genres Crassostrea et Saccostrea) afin d'acquérir des connaissances sur ces espèces et d'évaluer l'intérêt qu'elles pourraient présenter pour l'ostréiculture locale. L'importation d'espèces étrangères sur les côtes françaises est désormais strictement réglementée: la dissémination d'une nouvelle espèce peut se révéler néfaste d'un point de vue écologique et nuire aux productions existantes. De plus, ces huîtres peuvent être porteuses de pathogènes qui, s'il venaient à se répandre dans le milieu, pourraient toucher les stocks... |
| Tipo: Text |
Palavras-chave: Génétique; Acclimatation; Croisement; Crassostrea gigas; Crassostrea ariakensis; Crassostrea sikamea; Hybridation. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/00044/15514/12901.pdf |
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| Boudry, Pierre; Naciri, Yamama; Heurtebise, Serge; Delsert, Claude; Ledu, Christophe; Phelipot, Pascal; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre. |
| Le laboratoire IFREMER "Génétique Aquaculture et Pathologie" (GAP) (La Tremblade et Bouin) a entrepris des actions de recherche sur ces différents points. L'Unité de Recherche en Génétique, en collaboration avec tous les membres du Réseau Génétique Mollusques (REGEMO), a axé une partie de ses travaux sur l'acclimatation et l'hybridation de différentes espèces du genre Crassostrea. Ces recherches ont débuté avec le soutien du Conseil Général de Charente-Maritime depuis 1992 sur des essais d'acclimatation de C. virginica, l'huître creuse américaine. En prolongement de cette étude, l'URGE a proposé à la région Poitou-Charentes un programme de recherche plus vaste sur l'ensemble des espèces d'huîtres creuses. |
| Tipo: Text |
Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea virgnica. |
| Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00044/15515/12902.pdf |
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| Boudry, Pierre; Naciri, Yamama; Heral, Maurice; Collet, Bertrand; Bougrier, Serge; Geffard, Olivier; Ledu, Christophe; Phelipot, Pascal; Heurtebise, Serge; Gerard, Andre. |
| Le programme de recherche présenté ci-dessous est le fruit d'une réflexion conjointe entre généticiens et physiologistes afin de mettre sur pied un ensemble d'expériences permettant d'étudier les bases génétiques de la variabilité des caractères impliqués dans la croissance chez l'huître creuse, Crassostrea gigas. |
| Tipo: Text |
Palavras-chave: Génétique; Sélection génétique; Génétique des populations; Croissance; Crassostrea gigas. |
| Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00044/15518/12905.pdf |
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| Gagnaire, Pierre-alexandre; Lamy, Jean-baptiste; Cornette, Florence; Heurtebise, Serge; Degremont, Lionel; Flahauw, Emilie; Boudry, Pierre; Bierne, Nicolas; Lapegue, Sylvie. |
| The Pacific cupped oyster is genetically subdivided into two sister taxa, Crassostrea gigas and C. angulata, which are in contact in the north-western Pacific. The nature and origin of their genetic and taxonomic differentiation remains controversial due the lack of known reproductive barriers and the high degree of morphologic similarity. In particular, whether the presence of ecological and/or intrinsic isolating mechanisms contributes to species divergence is unknown. The recent co-introduction of both taxa into Europe offers a unique opportunity to test how genetic differentiation is maintained under new environmental and demographic conditions. We generated a pseudo-chromosome assembly of the Pacific oyster genome using a combination of BAC-end... |
| Tipo: Text |
Palavras-chave: Cupped oysters; Genome assembly; Species divergence; Reproductive barriers; Recombination rate. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00455/56622/58337.pdf |
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| Gagnaire, Pierre-alexandre; Cornette, Florence; Heurtebise, Serge; Auge, M. T.; Bierne, Nicolas; Lapegue, Sylvie. |
| The cupped oysters Crassostrea angulata and Crassostrea gigas have been sequentially introduced in Europe from their native Pacific area for aquaculture purposes. Ongoing viral epidemic disease is responsible for large losses in oyster aquaculture production throughout Europe. In parallel with current efforts in developing selection programs for disease resistance, it becomes critically important to characterize genetic diversity patterns in both native and introduced areas. With the advent of next generation sequencing techniques, the power to resolve the fine-scale genetic structure at a genome-wide scale opens to new perspectives towards genetic resource management. Here, we present a genome-wide screen of genetic variation that combines SNP genotyping... |
| Tipo: Text |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00167/27816/26009.pdf |
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| Ernande, Bruno; Boudry, Pierre; Heurtebise, Serge; Haure, Joel; Martin, Jean-louis. |
| Growth and survival are the two most interesting characters for oyster farming as they determine the biomass produced in the farms (growth x survival). Thus, a selection program aiming at improving these two characters could be of a great help. However, the oyster, as a sessile organism, is influenced by environmental variability (spatial and temporal) either natural or caused by the used oyster culture methods. Submitted to this variability, oysters must have evolved so as to adapt (short term) to this environmental variability. |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Huîtres; Croissance; Plasticité; Génétique. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3279.pdf |
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| Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
| Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the... |
| Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf |
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| Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumont, Andrew; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
| Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to... |
| Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4535.pdf |
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| Harrang, Estelle; Heurtebise, Serge; Faury, Nicole; Robert, Maeva; Arzul, Isabelle; Lapegue, Sylvie. |
| The present study identifies quantitative trait loci (QTLs) in response to an experimental infection with the parasite responsible for bonamiosis, Bonamia ostreae, in two segregating families of the European flat oyster, Ostrea edulis. We first constructed a genetic-linkage map for each studied family and improved the existing genetic-linkage map for the European flat oyster with a set of SNP markers. This latter map now combines the best accuracy and the best estimate of the genome coverage available for an oyster species. Secondly, by comparing the QTLs detected in this study with those previously published for O. edulis in similar experimental conditions, we identified several potential QTLs that were identical between the different families, and also... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Single Nucleotide Polymorphism; Disease resistance; Genetic and QTL mapping; Candidate genes. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00271/38244/36429.pdf |
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| Lallias, Delphine; Gomez-raya, L.; Haley, C. S.; Arzul, Isabelle; Heurtebise, Serge; Beaumont, A. R.; Boudry, Pierre; Lapegue, Sylvie. |
| We have identified quantitative trait loci (QTL) in the flat oyster (Ostrea edulis) for resistance to Bonamia ostreae, a parasite responsible for the dramatic reduction in the aquaculture of this species. An F-2 family from a cross between a wild oyster and an individual from a family selected for resistance to bonamiosis was cultured with wild oysters injected with the parasite, leading to 20% cumulative mortality. Selective genotyping of 92 out of a total of 550 F-2 progeny (i.e., 46 heavily infected oysters that died and 46 parasite-free oysters that survived) was performed using 20 microsatellites and 34 amplification fragment length polymorphism primer pairs. Both a two-stage testing strategy and QTL interval mapping methods were used. The two-stage... |
| Tipo: Text |
Palavras-chave: Statistical power; Survival analysis; QTL mapping; Disease resistance; Ostrea edulis. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6682.pdf |
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| Danic-tchaleu, Gwenaelle; Heurtebise, Serge; Morga, Benjamin; Lapegue, Sylvie. |
| Background Because of its typical architecture, inheritance and small size, mitochondrial (mt) DNA is widely used for phylogenetic studies. Gene order is generally conserved in most taxa although some groups show considerable variation. This is particularly true in the phylum Mollusca, especially in the Bivalvia. During the last few years, there have been significant increases in the number of complete mitochondrial sequences available. For bivalves, 35 complete mitochondrial genomes are now available in GenBank, a number that has more than doubled in the last three years, representing 6 families and 23 genera. In the current study, we determined the complete mtDNA sequence of Ostrea edulis, the European flat oyster. We present an analysis of features of... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00045/15628/13031.pdf |
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| Registros recuperados: 79 | |
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